Blar i forfatter "Roberts, Adam P."

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    • Capture of a novel, antibiotic resistance encoding, mobile genetic element from Escherichia coli using a new entrapment vector 

      Tansirichaiya, Supathep; Moyo, Sabrina John; Al-Haroni, Mohammed; Roberts, Adam P. (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-03-01)
      <b><p>Aims</b> Antimicrobial resistance genes (ARGs) are often associated with mobile genetic elements (MGEs), which facilitate their movement within and between bacterial populations. Detection of mobility is therefore important to understand the dynamics of MGE dissemination and their associated genes, especially in resistant clinical isolates that often have multiple ARGs associated with MGEs. ...

    • Characterisation of the Plasmidome within Enterococcus faecalis Isolated from Marginal Periodontitis Patients in Norway 

      Song, Xiaobo; Sun, Jinglu; Mikalsen, Theresa; Roberts, Adam P.; Sundsfjord, Arnfinn (Journal article; Tidsskriftartikkel; Peer reviewed, 2013)
      The present study aimed to identify and characterize plasmids in a national collection of oral Enterococcus faecalis (n = 106) isolated from patients with marginal periodontitis. Plasmid replicon typing was performed by multiplex-PCR and sequencing with specific primers for 18 rep-families and 1 unique sequence. Additional plasmid analysis by S1-PFGE was performed for comparison. Totally 120 plasmid ...

    • Detection of mobile genetic elements associated with antibiotic resistance in Salmonella enterica using a newly developed web tool: MobileElementFinder 

      Johansson, Markus H; Bortolaia, Valeria; Tansirichaiya, Supathep; Aarestrup, Frank M.; Roberts, Adam P.; Petersen, Thomas Nordahl (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-10-03)
      <i>Objectives</i> - Antimicrobial resistance (AMR) in clinically relevant bacteria is a growing threat to public health globally. In these bacteria, antimicrobial resistance genes are often associated with mobile genetic elements (MGEs), which promote their mobility, enabling them to rapidly spread throughout a bacterial community.<p> <i>Methods</i> - The tool MobileElementFinder was developed ...

    • Determination of copy number and circularization ratio of Tn916-Tn1545 family of conjugative transposons in oral streptococci by droplet digital PCR 

      Lunde, Tracy Munthali; Al-Haroni, Mohammed; Roberts, Adam P. (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-12-06)
      <p><I>Background</I>: Tn916 and Tn1545 are paradigms of a large family of related, broad host range, conjugative transposons that are widely distributed in bacteria and contribute to the spread of antibiotic resistance genes (ARGs). Variation in the copy number (CN) of Tn916-Tn1545 elements and the circularization ratio (CR) may play an important role in propagation of ARGs carried by these ...

    • Fitness costs of various mobile genetic elements in Enterococcus faecium and Enterococcus faecalis 

      Starikova, Irina; Al-Haroni, Mohammed; Werner, Guido; Roberts, Adam P.; Nielsen, Kåre M.; Johnsen, Pål Jarle (Journal article; Tidsskriftartikkel; Peer reviewed, 2013-07-05)
      Objectives: To determine the fitness effects of various mobile genetic elements (MGEs) in Enterococcus faecium and Enterococcus faecalis when newly acquired. We also tested the hypothesis that the biological cost of vancomycin resistance plasmids could be mitigated during continuous growth in the laboratory.<p> <p>Methods: Different MGEs, including two conjugative transposons (CTns) of the Tn916 ...

    • Intracellular Transposition and Capture of Mobile Genetic Elements following Intercellular Conjugation of Multidrug Resistance Conjugative Plasmids from Clinical Enterobacteriaceae Isolates 

      Tansirichaiya, Supathep; Goodman, Richard N.; Guo, Xinyu; Bulgasim, Issra; Samuelsen, Ørjan; Al-Haroni, Mohammed; Roberts, Adam P. (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-01-19)
      Mobile genetic elements (MGEs) are often associated with antimicrobial resistance genes (ARGs). They are responsible for intracellular transposition between different replicons and intercellular conjugation and are therefore important agents of ARG dissemination. Detection and characterization of functional MGEs, especially in clinical isolates, would increase our understanding of the underlying ...

    • Promoter activity of ORF-less gene cassettes isolated from the oral metagenome 

      Tansirichaiya, Supathep; Mullany, Peter; Roberts, Adam P. (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-06-10)
      Integrons are genetic elements consisting of a functional platform for recombination and expression of gene cassettes (GCs). GCs usually carry promoter-less open reading frames (ORFs), encoding proteins with various functions including antibiotic resistance. The transcription of GCs relies mainly on a cassette promoter (P<sub>C</sub>), located upstream of an array of GCs. Some integron GCs, called ...

    • The Transposon Registry 

      Tansirichaiya, Supathep; Rahman, Mohammad Ajijur; Roberts, Adam P. (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-10-09)
      Transposable elements in prokaryotes are found in many forms and therefore a robust nomenclature system is needed in order to allow researchers to describe and search for them in publications and databases. Here we provide an update on The Transposon Registry which allocates numbers to any prokaryotic transposable element. Additionally, we present the completion of registry records for all transposons ...